Sphingosylphosphorylcholine is a ligand for ovarian cancer G-protein-coupled receptor 1
- 4 April 2000
- journal article
- retracted article
- Published by Springer Nature in Nature Cell Biology
- Vol. 2  (5) , 261-267
- https://doi.org/10.1038/35010529
Abstract
Sphingosylphosphorylcholine (SPC) is a bioactive lipid that acts as an intracellular and extracellular signalling molecule in numerous biological processes. Many of the cellular actions of SPC are believed to be mediated by the activation of unidentified G-protein-coupled receptors. Here we show that SPC is a high-affinity ligand for an orphan receptor, ovarian cancer G-protein-coupled receptor 1 (OGR1). In OGR1-transfected cells, SPC binds to OGR1 with high affinity (Kd = 33.3 nM) and high specificity and transiently increases intracellular calcium. The specific binding of SPC to OGR1 also activates p42/44 mitogen-activated protein kinases (MAP kinases) and inhibits cell proliferation. In addition, SPC causes internalization of OGR1 in a structurally specific manner.Keywords
This publication has 48 references indexed in Scilit:
- Sphingosylphosphorylcholine Induces a Hypertrophic Growth Response Through the Mitogen-Activated Protein Kinase Signaling Cascade in Rat Neonatal Cardiac MyocytesCirculation Research, 1999
- Modulation of T-Lymphocyte Proliferation by Exogenous Natural Ceramides and SphingosylphosphorylcholineJournal of Investigative Dermatology Symposium Proceedings, 1999
- Sphingosylphosphorylcholine Stimulates Proliferation and Upregulates Cell Surface-Associated Plasminogen Activator Activity in Cultured Human KeratinocytesJournal of Investigative Dermatology, 1998
- Growth inhibition of human pancreatic cancer cells by sphingosylphosphorylcholine and influence of culture conditionsCellular and Molecular Life Sciences, 1997
- A New Wound Healing Agent—SphingosylphosphorylcholineJournal of Investigative Dermatology, 1996
- Sphingosylphosphorylcholine Activation of Mitogen-activated Protein Kinase in Swiss 3T3 Cells Requires Protein Kinase C and a Pertussis Toxin-sensitive G ProteinJournal of Biological Chemistry, 1995
- Sphingosylphosphorylcholine Rapidly Induces Tyrosine Phosphorylation of p125 and Paxillin, Rearrangement of the Actin Cytoskeleton and Focal Contact AssemblyPublished by Elsevier ,1995
- Lysophospholipids activate ovarian and breast cancer cellsBiochemical Journal, 1995
- Sphingolipid metabolites: Members of a new class of lipid second messengersThe Journal of Membrane Biology, 1995
- Sphingosylphosphorylcholine is a remarkably potent mitogen for a variety of cell linesBiochemical and Biophysical Research Communications, 1991