Chromosome location of the ribosomal RNA genes in Triturus vulgaris meridionalis (Amphibia, Urodela)

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Abstract
The mitotic chromosomes of six specimens from Triturus vulgaris meridionalis have been examined by both in situ hybridization with 3H 18S+28S rRNA and AS-SAT staining method. The results of these two sets of experiments can be summarized as follows: 1) in each specimen the NORs and the additional ribosomal sites, which react positively to in situ hybridization with 3H 18S + 28S rRNA, are also stained by silver; 2) other chromosomal regions, which do not hybridize in situ with 3H 18S+28S rRNA, are on the other hand stained by the AS-SAT method. These latter Ag-positive sites show a species-specific pattern of chromosomal distribution.