Interaction of a Non‐Histone Chromatin Protein (High‐Mobility Group Protein 2) with DNA
- 28 June 1975
- journal article
- Published by Wiley in European Journal of Biochemistry
- Vol. 54 (2) , 427-433
- https://doi.org/10.1111/j.1432-1033.1975.tb04153.x
Abstract
1. The interaction with DNA of the calf thymus chromatin non-histone protein termed the high-mobility group protein 2 has been studied by sedimentation analysis in the ultracentrifuge and by measuring the binding of the 125I-labelled protein to DNA. The results have been compared with those obtained previously by us [Eur. J. Biochem. (1974) 47, 263-270] for the interaction of high-mobility group protein 1 with DNA. Although the binding parameters are similar for these two proteins, high-mobility group protein 2 differs from high-mobility group protein 1 in that the former appears to change the shape of the DNA to a more compact form. 2. The molecular weight of high-mobility group protein 2 has been determined by equilibrium sedimentation and a mean value of 26 000 was obtained. 3. A low level of nuclease activity detected in one preparation of high-mobility group protein 2 has been investigated.Keywords
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