A Novel Fluorometric Method for DNA and RNA Determination
- 1 February 1997
- journal article
- Published by Taylor & Francis in Analytical Letters
- Vol. 30 (3) , 527-536
- https://doi.org/10.1080/00032719708001799
Abstract
A method for the determination of nucleic acids with methylene blue(MB) as a fluorescence probe was developed, based on the quenching of the fluorescence of MB in the presence of nucleic acids. The calibration graph was linear in the range of 0-40 mu g/ml for CT DNA and 0-55 mu g/ml for yeast RNA, the limit of detection was 28 ng/ml for CT DNA and 82 ng/ml for yeast RNA. CT DNA could be determined in the presence of 30% (w/w) yeast RNA and yeast RNA was determined when the content of CT DNA in synthetic samples was below 5% (w/w)Keywords
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