Synthesis of Tyrosine Labeled With C 14

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Abstract
Following cerebral concussion, the cerebrospinal fluid (CSF) was able to split nucleic acids, as shown spectrophotometrically by a decrease of its selective absorption in ultraviolet light. This finding was believed caused by the appearance of enzymatic substs. in the CSF. It was a question whether such substances diffusing into the subarachnoid space after concussion could act upon nuclear substs. in nerve cells, particularly their tigroid bodies. CSF''s were incubated with deparaffinized paraffin sections from normal spinal cords for 4-5 hrs. at 37[degree]C and then stained by the Nissl method. The fluids were acidified before incubn. by adding 0.05 ml. M acetate buffer soln. at pH 4.05-0.45 ml. CSF, so that less than 0.5 ml. CSF sufficed for the test. This left most Nissl bodies of the motor cells intact, but some CSF''s caused dissolution of these bodies in the anterior horn cells. These latter CSF''s decreased markedly the selective absorption of nucleic acids whereas the inactive CSF''s did not affect the nucleic acids, as detd. by spectrophotometry in ultraviolet light.

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