Glucose transporters in chromaffin cells: Subcellular distribution and characterization
Open Access
- 29 February 1988
- journal article
- Published by Wiley in FEBS Letters
- Vol. 229 (1) , 35-39
- https://doi.org/10.1016/0014-5793(88)80792-0
Abstract
The glucose transporter was identified and characterized by cytochalasin B binding in subcellular membrane fractions of chromaffin tissue. The binding was saturable with a K d of about 0.3 μM for each subcellular fraction. The B max capacity was 12–16 pmol/mg protein for enriched plasma membrane fractions, 6.3 pmol/mg protein for microsomal membrane preparations and 5.4 pmol/mg protein for chromaffin granule membranes. Irreversible photoaffinity labelling of the glucose‐protectable binding sites with [3H]cytochalasin B followed by solubilization and polyacrylamide gel electrophoresis from enriched plasma membrane preparations demonstrated the presence of three molecular species: 97±10, 51.5±6 and 30±4 kDa. The chromaffin granule membranes showed only a molecular species of 80±10 kDa.Keywords
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