A rapid polymerase chain reaction method for identifying fixed specimens

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Abstract
DNA profiling analysis of preserved tissue samples was achieved by using an extraction method which incorporated the use of a chelating resin “Chelex” to chelate inhibitory polyvalent metal ions. An automated sequencer was used to estimate sizes of low molecular weight trimeric and tetrameric microsatellites labelled with fluorescent dyes. The entire analysis can be completed within 48h. Probabilities of chance association using two microsatellites were calculated as ranging between 10−2and 10−4. The methods described have considerable potential for use in routine genetic analysis of preserved samples.