Multifunctional DNA conjugates for the in vitro selection of new catalysts
Open Access
- 15 April 2000
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 28 (8) , 35e-00
- https://doi.org/10.1093/nar/28.8.e35
Abstract
DNA–substrate conjugates are required for the direct in vitro selection of novel DNA catalysts for reactions between two small reactants. Here we describe the introduction of all necessary features into ssDNA by a novel, multifunctional primer containing a flexible PEG spacer, an o-nitrobenzyl moiety allowing for selective photocleavage, and anthracene as a reactant, a fluorescence label and/or an immobilization tag. These components were checked individually and by a mock selection.Keywords
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