Macrophage Colony‐stimulating Factor Induces the Growth and Differentiation of Normal Pregnancy Human Cytotrophoblast Cells and Hydatidiform Moles but Does Not Induce the Growth and Differentiation of Choriocarcinoma Cells

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Abstract
In the present study, we examined whether or not macrophage colony‐stimulating factor (M‐CSF; CSF‐1) is involved in the growth and differentiation of human chorionic, hydatidiform mole and choriocarcinoma cells. M‐CSF promotes the growth of early gestation chorionic cells, hydatidiform mole cells, and a human term placenta cell line (tPA30‐1). However, the growth of choriocarcinoma cells, BeWo, Jar, Jeg‐3, and NUC‐1, was not influenced at all by M‐CSF. M‐CSF promoted the secretion of human chorionic gonadotropin (hCG) and human placental lactogen (hPL), which are secreted from differentiated trophoblast, from early gestation chorionic cells and from hydatidiform mole cells. However, the secretion of hCG and hPL from choriocarcinoma cells was not affected by M‐CSF. When M‐CSF localization was examined by immunohistochemical staining, M‐CSF was detected in chorionic and hydatidiform mole cells, but was absent in choriocarcinoma cells. These results suggest that the growth and differentiation of normal chorionic and hydatidiform mole cells are M‐CSF‐dependent, while the growth and differentiation of choriocarcinoma cells are not.