Discovery and Directed Evolution of a Glyphosate Tolerance Gene
Top Cited Papers
- 21 May 2004
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 304 (5674) , 1151-1154
- https://doi.org/10.1126/science.1096770
Abstract
The herbicide glyphosate is effectively detoxified by N -acetylation. We screened a collection of microbial isolates and discovered enzymes exhibiting glyphosate N -acetyltransferase (GAT) activity. Kinetic properties of the discovered enzymes were insufficient to confer glyphosate tolerance to transgenic organisms. Eleven iterations of DNA shuffling improved enzyme efficiency by nearly four orders of magnitude from 0.87 mM –1 min –1 to 8320 mM –1 min –1 . From the fifth iteration and beyond, GAT enzymes conferred increasing glyphosate tolerance to Escherichia coli, Arabidopsis , tobacco, and maize. Glyphosate acetylation provides an alternative strategy for supporting glyphosate use on crops.Keywords
This publication has 15 references indexed in Scilit:
- Molecular Mechanism of the Enterococcal Aminoglycoside 6‘-N-Acetyltransferase‘: Role of GNAT-Conserved Residues in the Chemistry of Antibiotic InactivationBiochemistry, 2003
- Synthetic shuffling expands functional protein diversity by allowing amino acids to recombine independentlyNature Biotechnology, 2002
- Investigation of the Roles of Catalytic Residues in Serotonin N-AcetyltransferaseJournal of Biological Chemistry, 2002
- Tolerance and Accumulation of Shikimic Acid in Response to Glyphosate Applications in Glyphosate-Resistant and Nonglyphosate-Resistant Cotton (Gossypium hirsutum L.)Journal of Agricultural and Food Chemistry, 2001
- GCN5-Related N-Acetyltransferases: A Structural OverviewAnnual Review of Biophysics, 2000
- Gapped BLAST and PSI-BLAST: a new generation of protein database search programsNucleic Acids Research, 1997
- GCN5-related histone N-acetyltransferases belong to a diverse superfamily that includes the yeast SPT10 proteinTrends in Biochemical Sciences, 1997
- DNA shuffling by random fragmentation and reassembly: in vitro recombination for molecular evolution.Proceedings of the National Academy of Sciences, 1994
- Herbicide Resistance in Transgenic Plants Expressing a Bacterial Detoxification GeneScience, 1988
- Uptake and Distribution of N-Phosphonomethylglycine in Sugar Beet PlantsPlant Physiology, 1981