Immunohistochemical localization of fibroblast growth factor‐1 (FGF‐l)and FGF‐2 in cultured human ameloblastoma epithelial cells and ameloblastoma tissues

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Abstract
Fibroblast growth factor‐1 (FGF‐1) and FGF‐2 are mitogenic polypeptides that may contribute to neoplastic cell proliferation. In the present study, we established a serum‐free culture system for ameloblastoma cells and demonstrated that the addition of FGF‐1 and FGF‐2 enhanced cell growth in a dose‐dependent manner. Immunoperoxidase staining of cultured cells demonstrated strong expression of FGF‐1 and FGF‐2. In tissue specimens, FGF‐1 was localized in epithelial cell components of ameloblastomas, whereas FGF‐2 was mainly found in the basement membranes with only moderate staining in epithelium. These data suggest that both FGF‐1 and FGF‐2 may contribute to the growth and development of ameloblastomas.