INFLUENCE OF NON-STEROIDAL ANTI-INFLAMMATORY AGENTS ON LAPINE ARTICULAR CHONDROCYTE GROWTH-INVITRO

  • 1 January 1983
    • journal article
    • research article
    • Vol. 42  (2) , 58-65
Abstract
The lapine articular chondrocyte monolayer was used to study the effects on cell proliferation, morphology and synthesis of proteoglycans of 4 nonsteroidal antiinflammatory agents piroxicam, diclofenac sodium, sulindac sulfine and indomethacin. None of the substances caused any alteration to chondrocyte morphology. Cell proliferation and macromolecular binding of 35SO4 were unaffected by piroxicam (0.2, 2 and 20 .mu.g/ml) or diclofenac sodium (0.2, 2 and 5 .mu.g/ml). Whereas indomethacin markedly reduced cell proliferation (100 .mu.mol/l; P < 0.01), it failed to alter net proteoglycan synthesis significantly (1, 10 and 100 .mu.mol/l). Sulindac sulfide significantly reduced chondrocyte proliferation at 0.5 and 5 .mu.g/ml (P < 0.01). The 0.5 .mu.g/ml concentration also markedly stimulated proteoglycan synthesis (171%; P < 0.01). The use of the articular chondrocyte monolayer as an experimental model to investigate drug-induced alterations of cartilage metabolism is discussed.