Temperature‐dependent morphological changes in membranes of bacillus stearothermophilus

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Abstract
Bacillus stearothermophilus cells vary the lipid fatty acid composition of cytoplasmic membranes with growth temperature. Spin label studies of such membranes have been interpreted to indicate lateral lipid phase separations at the growth temperature. We have now used freeze‐fracture electron microscopy to confirm the spin label studies. Freeze‐fracture faces of protoplasts indicate slight but distinct protein aggregation at the growth temperature. Aggregation increases rapidly with decreasing quench temperature in wild‐type cells. In contrast we were unable to demonstrate extended protein segregation in membranes of a temperature‐sensitive mutant that contains more than 58% branched fatty acids.Storage of protoplasts for prolonged times below the lipid phase transition results in the appearance of corrugated fracture faces with 300‐ to 500‐Å repeat patterns, although this organism does not synthesize lecithins.