Immunofluorescence localization of proteins in semithin 0.2?1 ?m frozen sections of the ear
- 1 October 1981
- journal article
- research article
- Published by Springer Nature in European Archives of Oto-Rhino-Laryngology
- Vol. 233 (1) , 55-66
- https://doi.org/10.1007/bf00464275
Abstract
The present work describes a high resolution technique for locating proteins in frozen sections of the inner ear by immunofluorescence. Dissected organs are encapsulated in gelatin, and sections 0.1–1 μm thick are cut at −100°C in a cryoultramicrotome. These are labelled with antibodies against two cytoskeletal proteins, actin and tubulin. Actin, which had previously only been described in the sensory cells, is found in the supporting cells as well. Tubulin is identified in the supporting cells and in outer spiral nerve fibres. In dieser Arbeit wird eine Methode beschrieben, mit der man mit höherer Auflösung Proteine in Gefrierschnitten vom Innenohr durch Immunofluoreszens lokalisieren kann. Die Gewebestückchen werden in Gelatine eingebettet und Schnitte von 0,1–1 μm Dicke an einem Kryoultramikrotom bei −100° C geschnitten. Die Schnitte werden mit Antikörpern gegen die Proteine des Cytoskelets Aktin und Tubulin markiert. Actin, das bisher nur in der Sinneszelle lokalisiert war, wird nun auch in den Stützzellen gefunden. Tubulin wird in den Stützzellen und in den äußeren Spiralnervenfasern gefunden.Keywords
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