Transmural potential and stimulated acid in innervated rat stomach perfused at pH 3.0

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Abstract
Acid was secreted at high rates, reaching 5-13 ju.moles/min. This occurred promptly after subcutaneous injection of 400 ptg of a semipurified gastrin preparation using rats anesthetized with pento-barbital and prepared for a recirculation perfusion of the gastric lumen. The influent catheter entered via longitudinal incision of the esophageal wall, effluent via the pylorus. Only the inner epithelial tube of the esophagus was ligated to the entry catheter. Application of the "pH-stat" principle showed that stimulated acid secretion was sharply cut off when the perfusion medium was changed rapidly from pH 2.5 to pH 2.1. Sectioning the vagal trunks and their associated fibers in the forestomach area caused an immediate inhibition of stimulated acid secretion; this could not be restored by gastrin reinjection. Potential differences usually decreased from 41 to 36 mv between resting and stimulated acid rates, but this was not an invariable finding. Conductance and sec [short-circuit current] doubled under these conditions. Atropine at doses sufficient to block all receptor sites for inhibition of gastric potential had no effect on the stimulation of acid secretion by reinjection of gastrin.

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