Presence of cells in b‐cell lineage in mixed (GEMM) colonies from murine marrow cells

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Abstract
Recent progress in clonal cell culture techniques makes it possible to detect pluripotent hemopoietic precursors from murine marrow cells. The precursors can proliferate, differentiate and form mixed colonies containing eryth-roblasts, granulocytes, macrophages and often megakaryocytes in viscid culture medium. In the present investigation, the presence of cells of B-cell lineage in mixed colonies was investigated. Experiments on colonies containing clgM, clgG, slgM and slgG bearing cells using goat IgG fluorescein-conjugated anti-mouse IgM, goat F(ab′)2 fraction fluo-rescein-conjugated anti-mouse IgG and immunobeads revealed the presence of cytoplasmic IgM bearing cells in 47% of the colonies and surface IgM bearing cells in 74-84% of the colonies. Mixed colonies, however, did not contain either clgG bearing cells or slgG bearing cells. The results may indicate that some CFU-MIX proliferate and differentiate along B-cell lineage to slgM or clgM bearing cells in vitro.