Sensitivity of Cultured Tissue of Arabidopsis thaliana Races to Sulfanilamide

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Abstract
Callus of Arabidopsis thaliana (L.) Heynh. was examined for the kinetics of sulfanilamide inhibition of growth. Two geographic races of this species were compared for relative sulfanilamide sensitivity (as assessed by dry weight increase on drug‐containing medium) and rate of drug uptake.It was found that low concentrations of sulfanilamide in the culture medium (∼ 100 μM) inhibited callus growth and that low concentrations of p‐aminobenzoate (20 μM) competitively antagonized the sulfanilamide effect.Racial variation for sulfanilamide sensitivity was demonstrated. A partially drug‐resistant race had greater sulfanilamide uptake (per gram fresh weight) than a sensitive race. suggesting that relative drug‐sensitivity was not determined by the capacity to exclude sulfanilamide.