The membrane topology of EmrE – a small multidrug transporter from Escherichia coli
- 10 March 2004
- journal article
- Published by Wiley in FEBS Letters
- Vol. 562 (1-3) , 193-196
- https://doi.org/10.1016/s0014-5793(04)00240-6
Abstract
EmrE is a multidrug transporter from Escherichia coli that belongs to the Smr family of small multidrug transporters. The secondary structure of EmrE consists of a four helical bundle, as judged by different techniques. EmrE has been extensively characterized; nevertheless, the membrane topology of EmrE has not been determined yet. Previous work with a homologous Smr protein provided partial information of the membrane topology, however the location of the carboxy-terminus remained inconclusive. In this work we probed the membrane topology of EmrE, focusing on the carboxy-terminus of the protein, using two independent approaches. Our results support a secondary structure where the carboxy-terminus faces the cytoplasm, while the first loop faces the periplasmKeywords
This publication has 22 references indexed in Scilit:
- A major superfamily of transmembrane facilitators that catalyse uniport, symport and antiportPublished by Elsevier ,2002
- A Single Carboxyl Mutant of the Multidrug Transporter EmrE Is Fully FunctionalJournal of Biological Chemistry, 2001
- A Model for Coupling of H+ and Substrate Fluxes Based on “Time-Sharing” of a Common Binding SiteBiochemistry, 2000
- [8] Substituted-cysteine accessibility methodPublished by Elsevier ,1998
- Histidine 225, a Residue of the NhaA-Na+/H+ Antiporter of Escherichia coli Is Exposed and Faces the Cell ExteriorPublished by Elsevier ,1997
- Topological Analysis of NhaA, a Na+/H+ Antiporter from Escherichia coliJournal of Biological Chemistry, 1996
- P-glycoprotein and multidrug resistanceCurrent Opinion in Genetics & Development, 1996
- Determining the Secondary Structure and Orientation of EmrE, a Multi-Drug Transporter, Indicates a Transmembrane Four-Helix BundleBiochemistry, 1996
- Prevention of Drug Access to Bacterial Targets: Permeability Barriers and Active EffluxScience, 1994
- [26] Ion extrusion systems in Escherichia coliPublished by Elsevier ,1986