Improved determination of serum theophylline by gas chromatography with use of a nitrogen-phosphorus detector.
- 1 January 1979
- journal article
- research article
- Published by Oxford University Press (OUP) in Clinical Chemistry
- Vol. 25 (1) , 156-158
- https://doi.org/10.1093/clinchem/25.1.156
Abstract
We describe a method for the 7-min determination of theophylline in less than 100 muL of serum. The procedure requires no centrifugation or solvent evaporation after extraction. Butylation is done on the gas-chromatographic column by injecting the serum extract followed by a butylating mixture which contains 1-iodobutane as the alkylating agent. The method is precise, accurate, and free of interference. Results correlate well with those by ultraviolet spectrophotometry.This publication has 2 references indexed in Scilit:
- Gas-chromatographic micro-scale procedure for theophylline, with use of a nitrogen-sensitive detector.Clinical Chemistry, 1976
- Gas-chromatographic quantitation of theophylline in small volumes of plasma.Clinical Chemistry, 1976