Mutational mapping of RAS-responsive domains of the Saccharomyces cerevisiae adenylyl cyclase.
Open Access
- 1 June 1990
- journal article
- research article
- Published by Taylor & Francis in Molecular and Cellular Biology
- Vol. 10 (6) , 2539-2543
- https://doi.org/10.1128/mcb.10.6.2539
Abstract
Large deletion and small insertion mutations in the adenylyl cyclase gene of Saccharomyces cerevisiae were used to map regions required for activation by RAS protein in vitro. The amino-terminal 605 amino acids were found to be dispensable for responsiveness to RAS protein. All other deletions in adenylyl cyclase destroyed its ability to respond to RAS. Small insertion mutations within the leucine-rich repeat region also prevented RAS responsiveness, while other insertions did not.This publication has 26 references indexed in Scilit:
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