Laser Flow Cytofluorometric Analysis of Htc Cells Synchronized With Hydroxyurea, Nocodazole and Aphidicolin

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Abstract
The technique of laser flow cytofluorometry was used to monitor the arrival in G1 and the subsequent progression through the cell cycle of HTC [rat hepatoma] cells accumulated metaphase with colcemid alone or after treatment with hydroxyurea and nocodazole. Under the experimental conditions used, the latter procedure gave much better results, avoiding the extensive formation of micronucleated cells. Aphidicolin, an inhibitor of DNA polymerase, in combination with nocodazole, provides a useful method to tightly synchronize these cells at the G1/S border.