Development of sensitive methods for the detection of myobacteria by DNA probes
- 1 October 1990
- journal article
- Published by Oxford University Press (OUP) in FEMS Microbiology Letters
- Vol. 72 (1-2) , 29-33
- https://doi.org/10.1016/0378-1097(90)90339-r
Abstract
A sensitive method allowing the detection of myobacteria by DNA probing has been developed. Besides the choice of a relevant probe encoding for part of the ribosomal RNA genes, the critical step allowing this high sensitivity was the method by which mycobacteria were lysed. Sonication of mycobacteria in the presence of chloroform followed by dot blot by this lysate gave the highest sensitivity in the detection of sequences homologous to the DNA probes.Keywords
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