Phosphorothioate chemistry and its application to chemical synthesis of nucleic acids.

Abstract
Synthesis of phosphorothioates and their properties are reviewed from the standpoint of organophosphorus chemistry. Inherent synthetic problems in the P-S bond formation are described. Several methods for the synthesis of nucleoside phosphorothioates are introduced and their application to oligonucleotide synthesis is also described from various aspects. Especially, the versatile utility of the bis (phenylthio) phosphoryl group, which can be readily introduced to nucleoside hydroxyls, is shown with several successful examples of the synthesis of DNA and RNA fragments.

This publication has 0 references indexed in Scilit: