Studies of osmoregulation in salt adaptation of cyanobacteria with ESR spin-probe techniques

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Abstract
Sucrose is accumulated in response to NaCl-induced stress in the cyanobacterium Synechococcus 6311. Internal cell volume was measured by ESR spectra with 2,2,6,6-tetramethyl-4-oxopiperidinoxy free radical (TEMPONE) as a spin probe in order to calculate sucrose concentrations inside the cell. This method is rapid and reliable and provides an unambiguous measurement of absolute volumes in different osmotic environments. Because the osmolar concentration of sucrose does not counterbalance the osmolar concentrations of ions in the growth medium, sucrose accumulation may be one of the mechanisms involved in the process of adaptation to salt of Synchococcus 6311. The accumulation of sucrose in non-N2-fixing cyanobacteria such as Synechococcus 6311 and in N2-fixing cyanobacteria such as Nostoc muscorum suggests a common mechanism of osmoregulation of fresh water cyanobacteria in response to increasing NaCl concentrations in the growth medium.