Isolation and characterization of 45 polymorphic microsatellites from the bovine genome
- 1 February 1996
- journal article
- Published by Wiley in Animal Genetics
- Vol. 27 (1) , 43-47
- https://doi.org/10.1111/j.1365-2052.1996.tb01175.x
Abstract
A small‐insert bovine genomic library was constructed in pBluescript II SK(+) and enriched for microsatellites by selective rescue of single‐stranded pBluescript DNA carrying (CA)n/(TG) n tandem repeats. Approximately 50% of the clones in the enriched library contained (CA) nrepeats or CA‐rich sequences. Sequencing of clones selected for (CA) n repeats resulted in the identification and characterization of 45 (CA) npolymorphic microsatellites. Genotyping in 9 large paternal half‐sib families indicated that 40 of these microsatellite markers exhibit autosomal Mendelian inheritance. The numbers of alleles range from 2 to 18, with an average of 6‐3 per locus. The polymorphic microsatellite markers we have identified and characterized will contribute to the construction of a high‐resolution linkage map of bovine genome.Keywords
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