The cupric site in nitrous oxide reductase contains a mixed‐valence [Cu(II),Cu(I)] binuclear center: A multifrequency electron paramagnetic resonance investigation
- 19 December 1988
- journal article
- Published by Wiley in FEBS Letters
- Vol. 242 (1) , 70-74
- https://doi.org/10.1016/0014-5793(88)80987-6
Abstract
Multifrequency electron paramagnetic resonance (EPR) spectra of the Cu(II) site in nitrous oxide reductase (N2OR) from Pseudomonas stutzeri confirm the assignment of the low field g value at 2.18 consistent with the seven line pattern observed at 9.31 GHz, 10 K. S‐band spectra at 20 K are better resolved than the X‐band spectra recorded at 10 K. The features observed at 2.4, 3.4, 9.31 and 35 GHz are explained by a mixed‐valence [Cu(1.5)..Cu(1.5)] S= 1/2 species with the unpaired electron delocalized between two equivalent Cu nuclei. The resemblance of the N2OR S‐band spectra to the spectra for the EPR‐detectable Cu of cytochrome c oxidase suggests that the S‐band spectrum for cytochrome c oxidase measured below 30 K may also contain hyperfine splittings from two approximately equivalent Cu nuclei.Keywords
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