Genetical Analysis of a Sterile Mutant by Protoplast Fusion in the Fission Yeast Schizosaccharomyces pombe

Abstract
The genetical analysis, by protoplast fusion, of the sterile strain ED22 of S. pombe is described. Two major mutations are harbored by this strain: cdc 25.22, which is conditionally defective in mitosis; and, ste 1.1, which causes sterility in strains of h-, h+ or mat 2.102 mating-type. Sterility is due to the failure of cell agglutination. Mutations ste 1.1 is defective in the production of a nondiffusible and non-mating-type specific factor. Mutations ste 1 and cdc 25 both map on chromosome I and are loosely linked.

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