Parentage testing of dogs using variants of blood proteins: description of five new plasma protein polymorphisms
- 1 August 1987
- journal article
- research article
- Published by Wiley in Animal Genetics
- Vol. 18 (4) , 297-310
- https://doi.org/10.1111/j.1365-2052.1987.tb00773.x
Abstract
Summary. Plasma samples of 1126 dogs belonging to 21 different European breeds were analysed by two‐dimensional agarose gel (pH 5.4 or pH 8.6) – horizontal polyacrylamide gel (pH 9.0) electrophoresis, followed by general‐protein staining of gels. Genetic polymorphism was detected for five as yet unidentified proteins designated pretransferrin‐1 and ‐2 (Prt1 and Prt2) and postalbumin‐1, ‐2 and ‐3 (Pa1, Pa2 and Pa3). Three alleles are reported in the Prt1 and Pa2 systems and two alleles in the Prt2, Pa1 and Pa3 systems. While Prt2 variation was observed only in the cocker spaniel breed, each of the other four proteins showed a high degree of polymorphism in most of the breeds studied. Pa3 fractions were clearly observed only in samples stored at ‐20°C for more than 2 years. Prt1, Pa1 and Pa2 proteins are additional useful markers for parentage control in dogs. This study corroborated previously published results that dog plasma proteins, in general, show considerably more polymorphism than that reported for haemoglobin and for several blood cell enzymes in this species.Keywords
This publication has 31 references indexed in Scilit:
- Simultaneous phenotyping of pig plasma α-protease inhibitors (PI1, PO1A, PO1B, PI2) and four other proteins (PO2, TF, CP, HPX) by a simple method of 2D horizontal electrophoresisAnimal Genetics, 2009
- Prediction of the halothane (Hal) genotypes of pigs by deducing Hal, Phi, Po2, Pgd haplotypes of parents and offspring: results from a large‐scale practice in Swedish breedsAnimal Blood Groups and Biochemical Genetics, 1985
- A New Polymorphic Protein in Dog Serum Detected by SDS-Polyacrylamide Gel ElectrophoresisNihon Chikusan Gakkaiho, 1982
- Polymorphism of the third component of canine complementVeterinary Immunology and Immunopathology, 1981
- Exclusion probabilities obtainable by biochemical polymorphisms in dogsAnimal Blood Groups and Biochemical Genetics, 1981
- ESTERASE DTransplantation, 1980
- Studies of primate protein variation and evolution: Microelectrophoretic detectionBiochemical Genetics, 1980
- Genetic polymorphism of eserine resistant esterases in canine plasma*Animal Blood Groups and Biochemical Genetics, 1977
- GENETIC VARIATION OF RED BLOOD CELL ENZYMES IN THE DOGTransplantation, 1974
- ISOENZYME MARKERS IN DOG BLOOD CELLSTransplantation, 1973