ACTIONS OF RADIATIONS ON ENZYMES
- 1 July 1959
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Biochemistry
- Vol. 46 (7) , 925-932
- https://doi.org/10.1093/oxfordjournals.jbchem.a126984
Abstract
Yeast alcohol dehydrogenase and beef liver glutamic dehydrogenase (GD) were inactivated 50% by irradiation (with 104 and 105 r of gamma-rays) of 05- and 90-mg/100 ml solutions, respectively. The degree of inhibition was decreased to a certain limit with increasing dehydrogenase concentrations. Holoenzyme formed on adding diphosphopyridine nucleotide (DPN) was much less susceptible to gamma-ray inactivation than respective apoenzymes. DPN itself was shown to decay by gamma-ray irradiation. Reduced glutathione not only protected GD from gamma-ray inactivation but also partially restored the activity of gamma-ray-treated GD.Keywords
This publication has 1 reference indexed in Scilit:
- Actions of Radiations on EnzymesThe Journal of Biochemistry, 1959