Conversion of Androstenedione to 3β-Hydroxy-5-Androsten-17-One and 3β-Hydroxy-4-Androsten-17-One by the Testicular Microsomal Fraction of Sprague-Dawley Rats1
- 1 April 1987
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Biochemistry
- Vol. 101 (4) , 905-910
- https://doi.org/10.1093/oxfordjournals.jbchem.a121958
Abstract
When androstenedione was incubated with testicular microsomes of Sprague-Dawley rats in the presence of reduced nicotinamide-adenine dinucleotide (NADH), unknown metabolites were produced, in addition to testosterone and 7α-hydroxyandro-stenedione The metabolites were identified as 3β-hydroxy-4-androsten-17-one and 3β-hydroxy-5-androsten-17-one (3:1) by biochemical and radiochemical methods. These results confirmed the occurrence of the reverse reactions from androstenedione to 3β-hydroxy-5-androsten-17-one and 3β-hydroxy-5-androsten-17-one catalyzed by the 3β-hydroxysteroid dehydrogenase and 5-ene-4-ene isomerase in the microsomal fraction of Sprague-Dawley rat testes.This publication has 0 references indexed in Scilit: