Repair of DNA Double-Strand Breaks in Irradiated Yeast Cells under Nongrowth Conditions

Abstract
In diploid yeast radiation-induced DNA double-strand breaks can be repaired under nongrowth conditions at 30.degree. C and that liquid holding recovery of colony-forming ability is accompanied by a decrease in the number of double-strand breaks per cell. The kinetics of double-strand break repair under nongrowth conditions depend on the dose applied. For the highest dose used (2400 grays) repair is completed within about 48 h. Double-strand breaks which remain unrepaired after 48 h are irreparable under nongrowth conditions. The linear relationship between the initial number of double-strand breaks and dose is converted into a quadratic function of irreparable double-strand breaks with and dose during liquid holding treatment of cells.

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