Measurement of outward translocation of phospholipids across human erythrocyte membrane.
- 1 September 1988
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 85 (18) , 6783-6787
- https://doi.org/10.1073/pnas.85.18.6783
Abstract
Spin-labelled phospholipids have been used to study the outside .fwdarw. inside and inside .fwdarw. outside transport of phospholipids across the human erythrocyte membrane at 37.degree. C. As already shown, inward transport is much faster for aminophospholipids than for phosphatidycholine. In addition, we show here that outward transport of the phosphatidylserine and phosphatidylethanolamine analogues is three to four times faster than that of phosphatidycholine. Magnesium depletion of the erythrocytes considerably decreases the outward rate of both aminophospholipids to values close to that of phosphatidylcholine. These results suggest that the outward aminophospholipid translocation is, at least partly, protein mediated. The protein involved could be identical to the inward Mg-ATP-dependent aminophospholipid carrier.This publication has 14 references indexed in Scilit:
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