In vitro Sensitivity of Immature Human Mast Cells to Chemotherapeutic Agents

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Abstract
Cells from the human immature mast cell line, HMC-1 were tested for their sensitivity to 11 chemotherapeutic agents by changes in viability and incorporation of tritiated thymidine ([3H]-thymidine) after 72 h of in vitro drug exposure. Doxorubicin hydrochloride and cytosine arabinoside were the most active agents against HMC-1 cells at the concentrations tested. Doxorubicin hydrochloride inhibited the incorporation of [3H]-thymidine and decreased the viability of HMC-1 cells by > 90% at a concentration of 0.06 μg/ml. Cytosine arabinoside inhibited the incorporation of [3H]-thymidine and decreased the viability by > 95% at a concentration of 0.1 μg/ml. A clone of HMC-1 cells, A4, with an enhanced percentage (70–80%) of metachromatically staining cells was equally sensitive to these two agents; however, A4 cells were more sensitive to vinblastine sulfate and less sensitive to methylprednisolone than was the parent cell line. Both HMC-1 cells and A4 cells showed approximately equal sensitivity to etoposide and to mitomycin. These results show that human mast cells are susceptible in vitro to a number of commonly used chemotherapeutic drugs.

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