Isolation and Properties of Isolated Nuclei from the Florida Red Tide Dinoflagellate Gymnodinium breve (Davis)1
- 1 May 1982
- journal article
- research article
- Published by Wiley in The Journal of Protozoology
- Vol. 29 (2) , 217-222
- https://doi.org/10.1111/j.1550-7408.1982.tb04014.x
Abstract
A simple and rapid method is described for the isolation of nuclei from the Florida red tide dinoflagellate G. breve. The nuclei are free of cytoplasmic contamination and are active in endogenous RNA synthesis. The ratio of DNA:RNA:acid-soluble protein:acid-insoluble protein is 1:0.39:0.13:0.63, respectively, and each nucleus contains a .apprx. 113 pg of DNA. Electrophoretic analysis of the acid-soluble proteins reveals the presence of 2 histone-like proteins with MW of 12,000 and 13,000.Keywords
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