groELExpression ingyrBMutants ofBorrelia burgdorferi
- 1 November 2002
- journal article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 184 (21) , 6069-72
- https://doi.org/10.1128/jb.183.21.6069-6072.2002
Abstract
GroEL protein andgroELmRNA transcript were up-regulated ingyrBmutants ofBorrelia burgdorferi, a causative agent of Lyme disease. Furthermore, the protein and transcript levels ingyrBmutants were greater than those in experimentally heat-shocked cultures of wild-typeB. burgdorferi. Circular DNA in thegyrBmutants was more relaxed than in wild-type cells, althoughgroELis on the linear chromosome ofB. burgdorferi. To our knowledge, this is the first evidence, albeit indirect, for the effect of DNA topology on gene expression from a linear DNA molecule in a bacterium.Keywords
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