THE RELATION OF CEPHALIN FLOCCULATION AND COLLOIDAL GOLD REACTIONS TO THE SERUM PROTEINS 1

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Abstract
Electrophoretically separated [gamma] globulins from normal and pathological human sera show marked colloidal Au and cephalin flocculation activity. As little as 0.0002 to 0.001 mg. [gamma] globulin N gives a 5 reading in the colloidal Au test and 0.05 mg. [gamma] globulin N gives a definite cephalin flocculation. Addition of electrophoretically separated albumin to [gamma] globulin inhibits the colloidal Au reaction, but does not significantly inhibit the cephalin flocculation. Different preps. of albumin vary in inhibiting power. Sera with low electrophoretic A/G ratios show positive cephalin flocculation and colloidal Au reactions more frequently than those with normal A/G ratios, but other factors are also of importance.