Early events of in-vitro fertilization of cat eggs by epididymal spermatozoa

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Abstract
Summary. Newly ovulated eggs from mature queens treated with PMSG and hCG were inseminated in modified KRB solution with spermatozoa recovered from the cauda epididymidis of male cats. When 5 eggs were examined 15 min after insemination, no signs of sperm penetration into the vitellus were observed. However, in an egg examined before fixation 20 min after insemination, a spermatozoon whose head had passed through the zona pellucida was observed. Very high proportions (90– 100%) of the eggs were penetrated when they were examined 0·5–5 h after insemination. Male and female pronuclei were first observed in eggs examined 4 h after insemination.