Diverse Responses of Membrane-associated Enzymes of Ehrlich Ascites Tumor Cells to Anti-microtubular Agents

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Abstract
Ehrlich ascites tumor-bearing mice were treated with colchicine or mitomycin C. Under colchicine, adenylate cyclase activity in tumor cells decreased to approximately 1/3 of the untreated control level, whereas 5''-nucleotidase activity increased to almost twice the control level. Colchicine treatment resulted in about a 30% reduction of 3H-thymidine incorporation into DNA. Mitomycin C inhibited the incorporation of 3H-thymidine more markedly but changes in the 2 enzyme activities were less than under colchicine. The 2 enzyme activities and 3H-thymidine incorporation into DNA showed parallel changes with lapse of time after inoculation of tumor cells into mice. The colchicine effects on the 2 enzyme activities seemed not due to inhibition of cell proliferation. The 2 enzymes seemed to be associated differently with the microtubular system in Ehrlich ascites tumor cells.

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