Crystallization and preliminary X-ray diffraction studies of a rice cysteine proteinase inhibitor, Oryzacystatin-I.
- 1 April 1998
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Biochemistry
- Vol. 123 (4) , 568-570
- https://doi.org/10.1093/oxfordjournals.jbchem.a021974
Abstract
Oryzacystatin-I from rice seeds was overexpressed in Escherichia coli, purified, and crystallized by the sitting-drop vapor diffusion method. Crystals obtained with 2-methyl-2, 4-pentanediol as a precipitant exhibited space group I4122, with unit cell parameters of a= b = 100.0 ¨, c = 54.2 ¨, and diffracted up to 2.8 ¨ resolution at 100 K. The crystals have one molecule per asymmetric unit.Keywords
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