Structure and functional analysis of a marine bacterial carotenoid biosynthesis gene cluster and astaxanthin biosynthetic pathway proposed at the gene level
Open Access
- 1 November 1995
- journal article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 177 (22) , 6575-6584
- https://doi.org/10.1128/jb.177.22.6575-6584.1995
Abstract
A carotenoid biosynthesis gene cluster for the production of astaxanthin was isolated from the marine bacterium Agrobacterium aurantiacum. This cluster contained five carotenogenic genes with the same orientation, which were designated crtW, crtZ, crtY, crtI, and crtB. The stop codons of individual crt genes except for crtB overlapped the start codons of the following crt genes. Escherichia coli transformants carrying the Erwinia uredovora carotenoid biosynthesis genes provide suitable substrates for carotenoid biosynthesis. The functions of the five crt genes of A. aurantiacum were determined through chromatographic and spectroscopic analyses of the pigments accumulated in some E. coli transformants carrying various combinations of the E. uredovora and A. aurantiacum carotenogenic genes. As a result, the astaxanthin biosynthetic pathway is proposed for the first time at the level of the biosynthesis genes. The crtW and crtZ gene products, which mediated the oxygenation reactions from beta-carotene to astaxanthin, were found to have low substrate specificity. This allowed the production of many presumed intermediates of astaxanthin, i.e., adonixanthin, phoenicoxanthin (adonirubin), canthaxanthin, 3'-hydroxyechinenone, and 3-hydroxyechinenone.Keywords
This publication has 62 references indexed in Scilit:
- Cloning and expression inEscherichia coli of the gene encoding β‐C‐4‐oxygenase, that converts β‐carotene to the ketocarotenoid canthaxanthin inHaematococcus pluvialisPublished by Wiley ,2000
- Canthaxanthin Biosynthesis by the Conversion of Methylene to Keto Groups in a Hydrocarbon β-Carotene by a Single GeneBiochemical and Biophysical Research Communications, 1995
- Okadaxanthin, a novel C50-carotenoid from a bacterium,Pseudomonas sp. KK10206C associated with marine sponge,Halichondria okadaiCellular and Molecular Life Sciences, 1994
- Expression of the Genes Encoding the Early Carotenoid Biosynthetic-Enzymes in Capsicum annuumBiochemical and Biophysical Research Communications, 1993
- Cloning and functional expression in Escherichia coli of a cyanobacterial gene for lycopene cyclase, the enzyme that catalyzes the biosynthesis of β‐caroteneFEBS Letters, 1993
- In vitro expression and activity of lycopene cyclase and β‐carotene hydroxylase from Erwinia herbicolaFEBS Letters, 1993
- Molecular cloning and expression in Escherichia coli of a cyanobacterial gene coding for phytoene synthase, a carotenoid biosynthesis enzymeFEBS Letters, 1992
- Studies of immunomodulating actions of carotenoids. I. Effects ofβ‐carotene and astaxanthin on murine lymphocyte functions and cell surface marker expression inin vitroculture systemNutrition and Cancer, 1991
- Pigmentation of juvenile coho salmon with carotenoid oil extracted from Antarctic krillAquaculture, 1987
- Pigmentation of cultured red sea bream with astaxanthin diester purified from krill oil.NIPPON SUISAN GAKKAISHI, 1983