The Content of Dityrosine in Chick and Rabbit Aorta Proteins

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Abstract
The possible presence of dityrosine in elastin derived by 2 different methods and in structural glycoproteins from aortas of 1 day old chicks, adult rabbits and fetal rabbits was determined by a sensitive spectrofluorimetric procedure. Only chick tissues contained dityrosine, 0.3 residues/100,000 total amino acid residues in aortic elastin and 12-15 residues/100,000 residues in structural glycoproteins. No dityrosine was detected in any fetal or mature rabbit tissues. Related fluorescent compounds with different excitation-emission maxima and different elution times were obtained by ion exchange chromatography of structural glycoproteins partially hydrolyzed under alkaline conditions.