REQUIREMENT OF SPECIFIC ANTIBODY FOR KILLING OF ESCHERICHIA-COLI BY ALTERNATE COMPLEMENT PATHWAY IN BOVINE SERUM

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  • 1 January 1978
    • journal article
    • research article
    • Vol. 34  (1) , 131-136
Abstract
Experiments using the selective divalent cation chelator, ethylene glycol tetraacetic acid (EGTA) showed that the alternate complement [C] pathway was involved in the bactericidal action of bovine serum on 2 strains of Escherichia coli. The bactericidal system was, in experiments involving EGTA and .epsilon.-amino caproic acid, independent of Ca2+ and C1 [1st component of complement]. The fixation of C3-C9 by endotoxin does not require a functional classical pathway (C1, C4 and C2). In the case of 1 coliform strain (W1) however, there was an almost total dependence on a factor absorbable at 0.degree. C with homologous bacteria, and which appeared to be a natural antibody. In certain circumstances in the cow, .gamma. globulins may be directly involved in the activation of the alternate C pathway.