CONVERSION OF [1‐14C]PALMITIC ACID TO [1‐14C]HEXADECANOL BY DEVELOPING RAT BRAIN CELL‐FREE PREPARATIONS
- 1 January 1976
- journal article
- research article
- Published by Wiley in Journal of Neurochemistry
- Vol. 26 (1) , 107-113
- https://doi.org/10.1111/j.1471-4159.1976.tb04443.x
Abstract
—The conversion of [l‐14C]palmitic acid to [1‐14C]hexadecanol has been demonstrated with a cell‐free system from developing rat brain. ATP, Coenzyme A and Mg2+ were required for the activity. Fatty aldehyde was found to be an intermediate in this reaction. The conversion of fatty acid to fatty alcohol was mainly localized in the microsomal fraction and the formation of hexadecanol showed absolute specificity towards NADPH while fatty aldehyde was formed even in the absence of exogenous reduced pyridine nucleotides. The brain microsomes showed maximal activity with stearic acid and the activities with palmitic and oleic acids were 65% and 38% respectively of that with stearic acid. This enzymic reduction increased with age and showed a maximum in the 15‐day old rat brain.This publication has 51 references indexed in Scilit:
- A new procedure for the extraction, purification and fractionation of brain gangliosidesBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1973
- Synthesis of alcohol and wax ester by a cell-free system in Mycobacterium tuberculosisBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1972
- The incorporation of 18O and 14C from long-chain alcohols into the alkyl and alk-1-enyl ethers of phospholipids of developing rat brainBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1971
- The effect of Δ1-tetrahydrocannabinol on serotonin metabolism in the rat brainLife Sciences, 1971
- Enzymic interconversion of fatty alcohols and fatty acidsBiochemical and Biophysical Research Communications, 1970
- THE REGIONAL DISTRIBUTION OF SIALOGLYCOPROTEINS, GANGLIOSIDES AND SIALIDASE IN BOVINE BRAINJournal of Neurochemistry, 1970
- Biosynthesis of alkyl-ether containing lipid from dihydroxyacetone phosphateBiochemical and Biophysical Research Communications, 1969
- The biosynthesis of alkyl ether bonds in lipids by a cell-free systemBiochemical and Biophysical Research Communications, 1969
- The synthesis of glyceryl ether glycerol in Tetrehymena pyriformisBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1968
- A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATIONCanadian Journal of Biochemistry and Physiology, 1959