Histone‐Hydrocarbon Interaction. Partition of Histones in Aqueous Two‐Phase Systems Containing Poly(ethylene glycol)‐Bound Hydrocarbons

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Abstract
The hydrophobic properties of histones have been examined with help of the two-phase partition technique using dextran-poly(ethylene glycol)-water systems. We have found that different fatty acid esters of poly(ethylene glycol) interact with total histones in a manner similar to proteins of the type β-lactoglobulin and serum albumins. Thus the maximum interaction occurs when the fatty acid contains 16–18 carbon atoms. With less than eight carbon atoms in the polymer-bound fatty acid, no histone-hydrocarbon interaction is observed. The interaction of the five individual histone fractions with palmitate depends on the type of salt used and on its concentration. We suggest that the histones can be divided into three groups with decreasing hydrophobic properties: H3, H2a > H4, H2b > H1.