Evidence for glycosyl-phosphatidylinositol anchoring of Toxoplasma gondii major surface antigens.
Open Access
- 1 October 1989
- journal article
- research article
- Published by Taylor & Francis in Molecular and Cellular Biology
- Vol. 9 (10) , 4576-4580
- https://doi.org/10.1128/mcb.9.10.4576
Abstract
The four major surface antigens of Toxoplasma gondii tachyzoites (P43, P35, P30, and P22) were made water soluble by phosphatidylinositol-specific phospholipase C (PI-PLC). These antigens were biosynthetically labeled with 3H-fatty acids, [3H]ethanolamine, and [3H]carbohydrates. Treatment of 3H-fatty-acid-labeled parasite lysates with PI-PLC removed the radioactive label from these antigens. A cross-reacting determinant was exposed on these antigens after PI-PLC treatment.This publication has 27 references indexed in Scilit:
- Characterization of the cross‐reacting determinant (CRD) of the glycosyl‐phosphatidylinositol membrane anchor of Trypanosoma brucei variant surface glycoproteinEuropean Journal of Biochemistry, 1988
- Cell membrane, but not circulating, carcinoembryonic antigen is linked to a piiosphatidylinositol-containing hydrophobic domainBiochemical and Biophysical Research Communications, 1988
- CELL-SURFACE ANCHORING OF PROTEINS VIA GLYCOSYL-PHOSPHATIDYLINOSITOL STRUCTURESAnnual Review of Biochemistry, 1988
- Complete structure of the glycosyl phosphatidylinositol membrane anchor of rat brain Thy-1 glycoproteinNature, 1988
- Eukaryotic protein modification and membrane attachment via phosphatidylinositolCell, 1987
- Decay accelerating factor of complement is anchored to cells by a C-terminal glycolipidBiochemistry, 1986
- Immunological evidence of a common structure between Paramecium surface antigens and Trypanosoma variant surface glycoproteinsExperimental Cell Research, 1986
- A Glycophospholipid Tail at the Carboxyl Terminus of the Thy-1 Glycoprotein of Neurons and ThymocytesScience, 1985
- Crossreacting determinants in the C-terminal region of trypanosome variant surface antigensNature, 1979
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970