Simple Algorithm for Identification of Bordetella pertussis Pertactin Gene Variants
Open Access
- 1 April 2004
- journal article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 42 (4) , 1614-1619
- https://doi.org/10.1128/jcm.42.4.1614-1619.2004
Abstract
Studies performed in several countries have demonstrated the recent emergence and subsequent dominance of circulating Bordetella pertussis strains harboring pertactin and pertussis toxin variants not included in pertussis vaccines. Determination of the pertactin gene variants is commonly performed using a time-consuming and expensive sequence analysis. We developed a simple and reliable pertactin typing algorithm suitable for large-scale screening. The assay correctly identified all pertactin alleles in representative strains. The typing of 231 clinical strains of B. pertussis routinely isolated in Belgium showed that this algorithm was adequate to identify less-frequent prn types like prn9 and prn11 .Keywords
This publication has 14 references indexed in Scilit:
- Molecular Typing of Bordetella pertussis Isolates Recovered from Belgian Children and Their Household MembersClinical Infectious Diseases, 2003
- Systematic review of the effects of pertussis vaccines in childrenVaccine, 2003
- Multilocus Sequence Typing of Bordetella pertussis Based on Surface Protein GenesJournal of Clinical Microbiology, 2002
- Polymorphism of Bordetella pertussis Isolates Circulating for the Last 10 Years in France, Where a Single Effective Whole-Cell Vaccine Has Been Used for More than 30 YearsJournal of Clinical Microbiology, 2001
- Rapid Identification of Bordetella pertussis Pertactin Gene Variants Using LightCycler Real-Time Polymerase Chain Reaction Combined with Melting Curve Analysis and Gel ElectrophoresisEmerging Infectious Diseases, 2001
- Genotypic Variation in the Bordetella pertussis Virulence Factors Pertactin and Pertussis Toxin in Historical and Recent Clinical Isolates in the United KingdomInfection and Immunity, 2001
- Polymorphism inBordetella pertussisPertactin and Pertussis Toxin Virulence Factors in the United States, 1935–1999The Journal of Infectious Diseases, 2000
- Reemergence of Pertussis in the Highly Vaccinated Population of The Netherlands: Observations on Surveillance DataEmerging Infectious Diseases, 2000
- Antigenic variants in Bordetella pertussis strains isolated from vaccinated and unvaccinated childrenMicrobiology, 1999
- Changing Epidemiology of Pertussis in the United States: Increasing Reported Incidence Among Adolescents and Adults, 1990‐1996Clinical Infectious Diseases, 1999