Structure of spheroidene in the photosynthetic reaction center from YRhodobacter sphaeroides
- 12 November 1989
- journal article
- Published by Wiley in FEBS Letters
- Vol. 258 (1) , 47-50
- https://doi.org/10.1016/0014-5793(89)81612-6
Abstract
The structure of the reaction center of YRhodobacter sphaeroideshas been solved at 3 Å resolution, using the atomic coordinates of the reaction center from the carotenoidless mutant R26Rhodobacter sphaeroides. The structure has been refined by a simulated annealing with the computer program X‐PLOR, leading to a crystallographic R factor of 0.22 using reflections between 8 and 3 Å. The spheroidene molecule which is bound to the Y reaction center has been fitted in the electron density map as a 15‐cisisomer with a highly asymmetric structure. Thecis‐bond is located at proximity from ring I of the accessory bacteriochlorophyll on the inactive M side. The nature of thecis‐bond was confirmed by resonance Raman spectra obtained from Y reaction center crystals. The structure of spheroidene in Y reaction center is compared to that proposed for 1,2‐dihydroneurosporene inRhodopseudomonas viridisreaction center crystals.Keywords
This publication has 14 references indexed in Scilit:
- Structure of the reaction center from Rhodobacter sphaeroides R-26 and 2.4.1: protein-cofactor (bacteriochlorophyll, bacteriopheophytin, and carotenoid) interactions.Proceedings of the National Academy of Sciences, 1988
- Spectroscopic characterization of reaction center crystals from the carotenoid-containing wild-type strain Rhodobacter sphaeroides YFEBS Letters, 1988
- Crystallographic refinement by simulated annealingJournal of Molecular Biology, 1988
- The stereoisomerism of bacterial, reaction-center-bound carotenoids revisited: An electronic absorption, resonance Raman and 1H-NMR studyBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1987
- Preliminary characterization by X-ray diffraction of crystals of photochemical reaction centres from wild-type Rhodopseudomonas spheroidsJournal of Molecular Biology, 1987
- Structure of Rhodopseudomonas sphaeroides R‐26 reaction centerFEBS Letters, 1986
- Primary structure of the reaction center from Rhodopseudomonas sphaeroidesProteins-Structure Function and Bioinformatics, 1986
- Manganese‐quinone interactions in the electron acceptor region of bacterial photosynthetic reaction centresFEBS Letters, 1985
- On the state of carotenoids bound to reaction centers of photosynthetic bacteria: A resonance raman studyBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1978
- The protein data bank: A computer-based archival file for macromolecular structuresJournal of Molecular Biology, 1977