Improvement of Plasma Quality as Raw Material for Factor VIII:C Concentrates

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Abstract
Blood collected into different anticoagulants was stored in small tubes at +4.degree. C for up to 26 h. Seven blood coagulation analyses were performed under standardized conditions. High yield and stability of factor VIII:C [F VIII:C] were found for ACD [acid citrate detrose] and CPD[citrate-phosphate dextrose]-adenine. No changes could be found in the other 6 parameters tested. Whole blood in blood bags could be stored for 2-4 h at +4.degree. C with maximal yield of F VIII:C, with blood stored overnight the recovery was 65%. In plasma F VIII:C was stable for at least 2 h at room temperature. F VIIIR:Ag [factor VIII R antigen] and F VIIIR:RCoF were stable in both whole blood and plasma. No activation by plasmin as measured by B.beta. 15-42 could be demonstrated. The initial FPA [fibrinopeptide] levels, reflecting thrombin activation, in the donated blood differed individually and in some blood bags very high concentrations were found. The levels of FPA were not correlated to the time for collection of a bag of blood.