Regulation of Adenylate Cyclase and Cyclic-AMP Dependent Protein Kinase Activities in A2-Cell Rich Guinea-Pig Islets of Langerhans
- 1 January 1974
- journal article
- research article
- Published by Georg Thieme Verlag KG in Hormone and Metabolic Research
- Vol. 6 (01) , 49-52
- https://doi.org/10.1055/s-0028-1093883
Abstract
Islets of Langerhans isolated from streptozotocin-treated guinea-pigs contain a majority of glucagon-producing A2-cells. These islets have been used to study the activities of adenylate cyclase and protein kinase in the pancreatic A2-cells. Both enzymes were present in the A2-cell richt islets and possessed general characteristics similar to those of B-cell rich islet tissue. The A2-cell adenylate cyclase was stimulated by adrenaline, and the protein kinase was stimulated in the presence of adrenaline and theophylline, agents which also increased glucagon release from guinea-pig islets. A major difference was therefore noted between the responses of the A2-cells and B-cells to adrenaline, since adrenaline decreases the activity of adenylate cyclase and protein kinase in the B-cells. Nevertheless, both cell types responded to an elevation of adenylate cyclase and protein kinase activity with an increase in hormone secretion.Keywords
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