Chromosome organization of Streptococcus mutans GS-5
- 1 January 1993
- journal article
- research article
- Published by Microbiology Society in Journal of General Microbiology
- Vol. 139 (1) , 67-77
- https://doi.org/10.1099/00221287-139-1-67
Abstract
Twenty-eight genetic loci have been physically mapped to specific large restriction fragments of the Streptococcus mutans GS-5 chromosome by hybridization with probes of cloned genes or, for transposon-generated amino acid auxotrophs, with probes for Tn916. In addition, restriction fragments generated by one low-frequency-cleavage enzyme were used as probes to identify overlapping fragments generated by other restriction enzymes. The approach allowed construction of a low resolution physical map of the S. mutans GS-5 genome using restriction enzymes ApaI (5'-GGGCC/C), SmaI (5'-CCC/GGG), and NotI (5'-GC/GGCCGC).Keywords
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